——测量小的叶片与难以测量样品叶绿素含量的新方法

一、概述

CCM-300是使用叶绿素荧光比率对叶绿素含量测量的一种新型仪器。与传统的化学测量方法和吸收性测量方法相比，它可以对非常小的叶片以及难以测量样品的叶绿素含量进行非破坏性测量，测量迅速可靠。

二、用途

广泛应用于植物生理生态研究，特别是对传统吸收法测量困难的小叶片或样品的叶绿素含量进行测量，例如针叶、发育未完全的水稻、生于岩石上的丝状藻、地衣、草坪草、仙人掌、龙舌兰属植物、菠萝、拟南芥、果实、苔藓、叶茎、叶柄。

三、原理

尽管实践已经证明了吸收技术测量叶绿素含量的有效性，但该方法对样品的要求较高，使其应用范围受到限制。而样品本身的叶绿素荧光则不受样品厚度、均一性等的影响，可以指示叶绿素的含量。根据这个原理，Gitelson等比较了叶片的吸收和反射对叶绿素荧光的影响，发现在735nm和700nm处的比值与叶片的反射相关性最高，并且当样品叶绿素含量在41 mg· m^-2到675 mg· m^-2时，F735/F700的比值与其叶绿素含量间存在着显著的线性关系（R²≥0.95），与传统的吸收性测量方法相比（测量范围≤300 mg· m^-2），提高了叶绿素含量的线性关系范围。

根据Gitelson的研究结果，CCM-300使用具有15nm半峰宽的460nm二极管用于荧光激发，提高了红色荧光波长，避开了大约685nm的发射荧光峰（叶绿素可重新吸收，降低测量精度）。仪器使用在735nm和700nm的叶绿素发射荧光的比率（F735/F700）进行叶绿素含量的测量，读数为F735/F700的比值以及根据Gitelson方程计算的叶绿素含量。

四、特点

Ø 直接算出叶绿素含量mg/m²;

Ø 测量结果可靠，不受叶片或样品大小、厚度和形状的影响；

Ø 经济、轻便、手持式设计，适合野外使用；

Ø 与化学测量结果高度相关，即使在高叶绿素含量时；

Ø 彩色触摸屏显示；

Ø 离散的单点测量和多点(2-30个样品)平均值测量模式；

Ø 非易失性闪存（2GB）；

Ø USB数据输出，可使用Excel打开；

五、组成

主机、光纤样品夹、电池充电器、4节AA镍氢充电电池、USB通讯缆线、使用手册。

可选配件:

20mm大探头: 增加测量面积。 较大的样本量可能对某些类型的样本有用，例如苔藓;

六、技术参数

Ø 测量参数：叶绿素荧光比率——发射荧光在735nm/700nm的比值（CFR），或相对叶绿素含量mg· m^-2；

Ø 测量面积：直径3mm的圆，外径4mm，但对直径小于3mm的样品也可准确可靠的测量；

Ø 分辨率：比值0.01或1 mg· m^-2；

Ø 重复性：依赖于信号强度，对信号强度低的样品，建议使用多点测量的平均值，对信号强度高的样品，差异通常为±0.03或更低；。

Ø 噪声：<±0.02；

Ø 光源：LED 460 nm蓝光二极管，半峰宽15 nm；

Ø 探头：两个固态高感传感器，提供滤光片。同时提供两个波长探头（700nm~710nm，730nm~740nm）；

Ø 检测：数控调制光，将背景光影响将至最低。并对光源和检测器进行温度补偿；

Ø 存储容量：2GB；

Ø 模式：单点测量，2~30个点去平均值，去除2σ区间外的平均值，或者中位数；

Ø 样品接口：光纤传感器，直径4mm；

Ø 用户界面：240 × 320 像素彩色触摸屏；

Ø 输出：USB 1.1；

Ø 温度范围：0~50 ℃；

Ø 电源：2节充电AA电池；

Ø 自动关闭间隔：（无按键或下载）0~20分钟可选；

Ø 大小：12 cm × 9 cm × 3 cm；

Ø 重量：0.16lbs 275g；

Ø 测量时间：5秒；

六、产地:美国

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